ABSTRACT
This study was carried out to augment the colonization of marine benthic communities on artificial reef structure. Increasing marine pollution along with various natural hazards cause severe damages to marine algae and associated fauna. In recent years, artificial reefs have been deployed in coastal regions of several parts of the world in order to increase the marine productivity. They are mainly built with concrete materials, however their leachates have considerable impacts on algae. Therefore to increase the algal colonization five chemoattractants such as ferrous sulfate, zinc oxide, ammonium nitrate, sodium phosphate and ferrous lactate were screened against spores of a fouling alga, Ulva pertusa. FeSO4 / ZnO (8:2) and ferrous lactate coatings showed the highest spore attachment with 52 +/- 5.2 cm2 and 79.5 +/- 10.2 cm2 spores respectively (p<0.01). Furthermore using these chemoattractants, coating formulations were made and their performances were investigated at East coast (Ayajin harbor) and South coast (Meejo harbor) of Korea. A maximum fouling coverage (with green algae 25%, red algae 11.3% and brown algae 63.7%) was estimated from ferrous lactate coatings (p<0.01). Different composition of coating formulations and their chemoattractive properties were evaluated.
Subject(s)
Eukaryota/drug effects , Animals , Anthozoa , Biomimetics , Chemotactic Factors/chemistry , Coated Materials, Biocompatible/chemistry , Ferrous Compounds , Geography , Korea , Lactates , Marine Biology , Nitrates , Phosphates , Spores/drug effects , Zinc OxideABSTRACT
Algal spores respond to many environmental variables, especially to chemical "cues". This chemotactic response can be utilized to attract spores, thereby colonization of a new substrata is possible to be influenced. In this attempt, four chemoattractant candidates were screened against spores of Ulva pertusa to reveal their efficiencies. Attachment and subsequent germination of Ulva spores were effectively influenced by these chemoattractant candidates. In particular 100 microg cm2 of D-glucose coating was found to enhance spore attachment by > 150%. Furthermore, field investigations carried out with test panels, clearly indicate the chemoattractive properties of test coatings. In recent years, various anthropogenic activities and natural hazards cause detrimental impacts on the benthic algae and other fishery resources. Artificial reefs have been laid on many coastal regions to increase or restore marine resources. Chemoattractant coatings can be applied on artificial surfaces to increase the colonization of benthic forms. It also can be used in the mariculture devices. Influence of chemoattractants on Ulva spores and fouling biomass estimated on test panels are discussed.
Subject(s)
Adhesiveness/drug effects , Animals , Anthozoa , Biofilms , Biomass , Biomimetics , Chemotactic Factors/chemistry , Choline , Coated Materials, Biocompatible/chemistry , Glucose , Glycine , Marine Biology , Methanol , Microscopy, Fluorescence , Organic Chemicals/chemistry , Spores/drug effects , Time Factors , Ulva/drug effectsABSTRACT
Marine pollutants induce changes in microalgal metabolism. In this study effects of tributyl-tin chloride (TBTCl) on a marine microalga Tetraselmis suecica was studied. The changes induced by TBTCI on growth rate, viability and biochemicals were assessed. In acute exposure to TBTCl, EC50 estimated for 24 hr was 2.02 microg ml(-1), whereas total lethality was observed at 4 microg ml(-1). In chronic exposure to TBTCl, at higher concentrations (0.5-1 microg ml(-1)) growth rate, chlorophyll pigments, carbohydrate and protein contents were reduced. The results of this study indicate that TBTCl toxicity made drastic changes in growth and biochemical composition of T. suecica.
Subject(s)
Chlorophyta/drug effects , Carbohydrate Metabolism/drug effects , Chlorophyll/metabolism , Plant Proteins/metabolism , Trialkyltin Compounds/toxicity , Water Pollutants, Chemical/toxicityABSTRACT
In recent years, industrial pollutants and the mountain forest fire ashes released into seawater cause damage to the marine environment, mainly it reduces the algal productivity in the inter tidal region. To get recover from the stress due to pollutants and to increase the growth and development of biofouling algae (benthic organisms), Ecklonia cava extract was investigated for its biofouling attracting efficiency. Bioactive guided fractions of E. cava extract derived from column chromatography were tested against spore attachment of a fouling alga, Ulva pertusa. Fraction B showed increased spore attachment rate with a maximum of 92 +/- 5%. This fraction was further analysed on HPLC, GC-Mass and NMR, deduced as pentadecanoic acid.
Subject(s)
Phaeophyceae/chemistry , Biological Products/isolation & purification , Fatty Acids/isolation & purification , Methanol/chemistry , Spores/drug effects , Ulva/drug effectsABSTRACT
Screening of test chemicals or formulations for antifouling (AF) activity is important to get first hand information on their nontoxic repelling activities. Especially spores of a fouling alga, Ulva pertusa were used in this study to test the AF efficiency of five organic chemicals. Coatings made with 100 microg cm2 of citral and eugenol significantly inhibited the spore attachment. A low concentration (1 microg cm2) of solanesol exhibited effective AF activity against spore attachment. Spore germination was sensitive to different AF candidates screened in this study. Based on the attachment and germination response of Ulva pertusa spores, AF efficiency of five organic AF candidates is discussed.
Subject(s)
Acetates/pharmacology , Eugenol/pharmacology , Germination/drug effects , Monoterpenes/pharmacology , Spores/drug effects , Terpenes/pharmacology , Ulva/drug effectsABSTRACT
In copper based antifouling (AF) paints Cu (I) oxide was largely used as booster biocide. In this study effect of Cu (I) oxide on two marine microalgae, Tetraselmis suecica and Dunaliella tertiolecta was demonstrated. EC50 (96 hr) concentrations estimated for T. suecica and D. tertiolecta were 1.3 mg l(-1) and 1.34 mg l(-1), respectively. Copper (I) oxide induced changes in growth, chlorophyll, carbohydrate and protein contents were observed in T. suecica and D. tertiolecta. At low concentration of 0.0625 mg l(-1), 3-26% and 1-16% growth stimulation was observed in T. suecica and T. tertiolecta respectively. Increasing Cu (I) oxide concentrations proportionately decreased the carbohydrate and protein contents. This study clearly indicates the toxicity of excessive Cu (I) oxide on growth and biochemical compositions of T. suecica and D. tertiolecta.
Subject(s)
Eukaryota/drug effects , Copper/pharmacology , Marine BiologyABSTRACT
Toxic antifouling chemicals released into the seawaters leads to marine environmental degradation. In order to identify a nontoxic antifoulant, an assessment of antifouling (AF) efficacy of some AF candidates was made at Ayagin harbor, east coast of South Korea. In this static panel study conducted during October 2000-March 2001, some commercial antifoulants, seaweed and seagrass extracts were screened. On panel surfaces coated with a seaweed extract, Ishige okamurae exhibited effective AF activity. Ulva pertusa was encountered as a 'monospecific' fouler with fairly high fouling coverage on many of the test panel surfaces. In recent years the increased influx of inorganic pollutants in the coastal waters causes exorbitant growth of fouling marine algae found all along the Korean peninsula. Especially, a cosmopolitan ship fouling alga U. pertusa occur with high abundance. It was largely suggested that the proposed international ban on the toxic antifoulant tributyltin (TBT) had significant effect on the 'green tide' phenomenon occurring in different parts of the world. However, it appears that Korean scenario of 'green tide' is a localized. Antifouling efficacy of some AF coatings and fouling coverage of a green tide alga, U. pertusa are discussed.